develop-hplc-method SKILL.md skillA
develop-hplc-method from pjt222/agent-almanac is an agent skill: a SKILL.md folder an AI agent loads when a task calls for it.
mdr add pjt222/agent-almanac/develop-hplc-method@v1.0Pins v1.0 in mdr.lock. Pin the fingerprint instead.
- Audit grade
- A, 17 of 17 checks passed
- Kind
- SKILL.md skill
- Repository
- pjt222/agent-almanac
- Path
i18n/caveman-lite/skills/develop-hplc-method/SKILL.md- Versions recorded
- 3
- Latest version
- v1.0, committed Aug 17, 2026
- First version recorded
- Apr 19, 2026
- Size
- 14,094 bytes
- Repository stars
- 43
- License
- MIT
Develop a high-performance liquid chromatography method: define separation goals, select column chemistry and mobile phase, optimize gradient and flow conditions, choose the appropriate detector, and evaluate initial method performance for target analytes in solution or complex matrices.
Collected from public GitHub. Every version is saved exactly as it was and never changes. Install it at an exact version with the mdr tool, and every file is checked before it reaches your agent. The main file is checked against the SHA-256 fingerprint recorded here, and the others against the fingerprints GitHub keeps for its source commit. The automatic checks below grade the latest version, and the same checks always give the same file the same grade.
What the file says
# Develop an HPLC Method Systematic development of a high-performance liquid chromatography method covering mode selection, column chemistry, mobile phase and gradient design, flow and temperature optimization, detector choice, and iterative refinement for non-volatile, thermally labile, or polar analytes. ## When to Use - Analyzing compounds that are non-volatile, thermally labile, or too polar for GC - Developing a new HPLC-UV, HPLC-fluorescence, or LC-MS method from scratch - Adapting a literature or pharmacopeial HPLC method to a different column or instrument - Improving an existing method that suffers from poor resolution, long run times, or sensitivity issues - Selecting the appropriate chromatographic mode (reversed-phase, HILIC, ion-exchange, SEC, chiral) ## Inputs ### Required - **Target analytes**: Compound names, structures, molecular weights, pKa values, logP/logD - **Sample matrix**: Formulation, biological fluid, environmental extract, or neat solution - **Performance targets**: Required resolution, detection limits, quantitation range ### Optional - **Reference method**: Compendial or literature method to use as a starting point …
Read the whole file at its exact version.
How to install
mdr add pjt222/agent-almanac/develop-hplc-method@sha256:eb6b49969ac640d7The line at the top of this page pins the label v1.0, which is what most people want. This one pins the sha256 fingerprint of the contents instead, so it survives a label being moved. Either way the exact version is written to mdr.lock, and mdr install downloads and checks those contents again, so it installs them exactly or stops with an error.
[](https://markdownregistry.com/a/art_aqnjyzgfbqcr3fnw)
3 badge image fetches in 30 days. What the badge shows.
Versions
The registry has recorded 3 versions of this file, between Apr 19, 2026 and Aug 17, 2026, about one every 60 days.
| version | committed | commit | size | audit | |
|---|---|---|---|---|---|
| v1.0 latest | 2026-08-17 | a784899 | 14,094 B | A | view · diff |
| v1.0 | 2026-04-23 | eb36d45 | 14,065 B | A | view · diff |
| v1.0 | 2026-04-19 | e334ebd | 14,120 B | A | view |
Audit of the latest version
- pass: Frontmatter block present
- pass: Frontmatter declares a name
- pass: Frontmatter declares a description
- pass: Size between 200 bytes and 200 KB (14094 bytes)
- pass: No zero-width or bidi control characters
- pass: No instruction hidden inside an HTML comment
- pass: No link to an exfiltration or paste host
- pass: No credential-shaped string
- pass: No instruction to send local credentials anywhere
- pass: No text hidden with inline styles
- pass: No prompt-injection phrasing
- pass: No curl or wget piped into a shell
- pass: No recursive delete of root, home or parent
- pass: No instruction to read or print local credentials
- pass: No base64 blob over 200 characters
- pass: No link to a raw IP address
- pass: No script tag
Source
pjt222/agent-almanac · 43 stars · license MIT · pushed 2026-11-06 · branch main
API
GET https://markdownregistry.com/api/v1/artifacts/art_aqnjyzgfbqcr3fnw GET https://markdownregistry.com/api/v1/resolve?ref=pjt222/agent-almanac/develop-hplc-method GET https://markdownregistry.com/api/v1/blob/eb6b49969ac640d740949aa77c2c91997a6c87b71d42c0a0f45f05b66b254143
Your AI assistant does the legwork. You hear about the deals worth your word. Hand yours the standing instructions at modelranch.com and it joins the network that reads files like this one.
Guides: the SKILL.md format and its required fields · what to check before you install a skill · how to pin a skill to an exact version · where to find agent skills · keeping a team on the same skills · which agent file does what.