bio-clip-seq-ago-clip-mirna-targets SKILL.md skillA
bio-clip-seq-ago-clip-mirna-targets from fridrichmethod/awesome-skills is an agent skill: a SKILL.md folder an AI agent loads when a task calls for it.
mdr add fridrichmethod/awesome-skills/bio-clip-seq-ago-clip-mirna-targets@git:20260726.5bc48c3Pins git:20260726.5bc48c3 in mdr.lock. Pin the fingerprint instead.
- Audit grade
- A, 17 of 17 checks passed
- Kind
- SKILL.md skill
- Repository
- fridrichmethod/awesome-skills
- Path
skills/ago-clip-mirna-targets/SKILL.md- Versions recorded
- 3
- Latest version
- git:20260726.5bc48c3, committed Jul 26, 2026
- First version recorded
- May 20, 2026
- Size
- 19,981 bytes
- Repository stars
- 15
- License
- a license file GitHub does not identify
Identify direct miRNA-target interactions from AGO HITS-CLIP, AGO-CLEAR-CLIP (chimeric reads), HEAP (Halo-Ago2 mouse), chimeric eCLIP / miR-eCLIP (deep miRNA-target profiling), or CLASH using chimeric-read processing pipelines, seed-pairing analysis, and 3' auxiliary pairing rules. Use when distinguishing direct miRNA targets from indirect, integrating CLIP-derived target maps with TargetScan / miRDB / DIANA predictions, applying canonical 7mer-8mer seed matching with 3' UTR context, or…
Collected from public GitHub. Every version is saved exactly as it was and never changes. Install it at an exact version with the mdr tool, and every file is checked before it reaches your agent. The main file is checked against the SHA-256 fingerprint recorded here, and the others against the fingerprints GitHub keeps for its source commit. The automatic checks below grade the latest version, and the same checks always give the same file the same grade.
What the file says
## Version Compatibility Reference examples tested with: eCLIP pipeline (Yeo lab), chimeric eCLIP analysis scripts (Yeo lab), HEAP pipeline (Li 2020), Hyb pipeline (Travis 2014), TargetScanHuman 8.0, miRDB 6.0, samtools 1.19+, bedtools 2.31+, pyHyb 0.4+. Before using code patterns, verify installed versions match. If versions differ: - Python: `pip show <package>` then `help(module.function)` to check signatures - CLI: `<tool> --version` then `<tool> --help` to confirm flags If code throws unexpected errors, introspect the installed package and adapt the example to match the actual API rather than retrying. # AGO-CLIP and miRNA Target Identification **"Identify direct miRNA-target interactions experimentally"** -> Use Argonaute (AGO1-4) CLIP-seq variants to map miRNA-binding sites on mRNAs, then resolve which miRNA pairs with each site. Three approaches: (a) standard AGO-CLIP recovers AGO-bound sites but cannot say which miRNA; (b) chimeric methods (CLEAR-CLIP, chimeric eCLIP / miR-eCLIP) ligate the miRNA to its target during library prep, producing miRNA-mRNA chimeric reads that unambiguously assign miRNA-target pairs; (c) HEAP uses HaloTag-Ago2 for in vivo profiling. The… …
Read the whole file at its exact version.
How to install
mdr add fridrichmethod/awesome-skills/bio-clip-seq-ago-clip-mirna-targets@sha256:d6b78e7631922c07The line at the top of this page pins the label git:20260726.5bc48c3, which is what most people want. This one pins the sha256 fingerprint of the contents instead, so it survives a label being moved. Either way the exact version is written to mdr.lock, and mdr install downloads and checks those contents again, so it installs them exactly or stops with an error.
[](https://markdownregistry.com/a/art_4n5g4hk7lxnch63y)
2 badge image fetches in 30 days. What the badge shows.
Versions
The registry has recorded 3 versions of this file, between May 20, 2026 and Jul 26, 2026, about one every 33 days.
| version | committed | commit | size | audit | |
|---|---|---|---|---|---|
| git:20260726.5bc48c3 latest | 2026-07-26 | 5bc48c3 | 19,981 B | A | view · diff |
| git:20260528.89ddef6 | 2026-05-28 | 89ddef6 | 20,451 B | A | view · diff |
| git:20260520.e1c72b6 | 2026-05-20 | e1c72b6 | 20,277 B | A | view |
Audit of the latest version
- pass: Frontmatter block present
- pass: Frontmatter declares a name
- pass: Frontmatter declares a description
- pass: Size between 200 bytes and 200 KB (19981 bytes)
- pass: No zero-width or bidi control characters
- pass: No instruction hidden inside an HTML comment
- pass: No link to an exfiltration or paste host
- pass: No credential-shaped string
- pass: No instruction to send local credentials anywhere
- pass: No text hidden with inline styles
- pass: No prompt-injection phrasing
- pass: No curl or wget piped into a shell
- pass: No recursive delete of root, home or parent
- pass: No instruction to read or print local credentials
- pass: No base64 blob over 200 characters
- pass: No link to a raw IP address
- pass: No script tag
Source
fridrichmethod/awesome-skills · 15 stars · license a license file GitHub does not identify · pushed 2026-10-18 · branch main
API
GET https://markdownregistry.com/api/v1/artifacts/art_4n5g4hk7lxnch63y GET https://markdownregistry.com/api/v1/resolve?ref=fridrichmethod/awesome-skills/bio-clip-seq-ago-clip-mirna-targets GET https://markdownregistry.com/api/v1/blob/d6b78e7631922c07de5e4cd92d0594eb63e9c5b1c7c0ad22989e18957eb88903
Your AI assistant does the legwork. You hear about the deals worth your word. Hand yours the standing instructions at modelranch.com and it joins the network that reads files like this one.
Guides: the SKILL.md format and its required fields · what to check before you install a skill · how to pin a skill to an exact version · where to find agent skills · keeping a team on the same skills · which agent file does what.
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