Home / ammawla / encode-toolkit · plugin/skills/cellxgene-context/SKILL.md · GitHub

cellxgene-context skillA

cellxgene-context is agent-read markdown (skill) from ammawla/encode-toolkit: Guide for integrating CellxGene Census single-cell data with ENCODE bulk experiments. Use when users need cell-type-specific expression context for ENCODE regulatory data, want to deconvolve bulk ENCODE signals, or validate regulatory elements at single-cell resolution. Trigger on: CellxGene, single-cell atlas, cell type expression, Census, cell type specificity, single-cell context, scRNA-seq atlas..

Indexed from public GitHub and served as immutable, content-addressed versions. Install it pinned to an exact SHA-256 with the mdr CLI, and every file is verified against the hash recorded here before it reaches your agent. The deterministic audit below grades the latest version, and the same file always earns the same grade.

What the file says

# Integrating CellxGene Census Single-Cell Data with ENCODE Bulk Experiments

Bridge bulk ENCODE functional genomics data with cell-type-specific expression from the CellxGene Census, the largest unified single-cell RNA-seq atlas, to resolve cell-type contributions to regulatory element activity.

## Scientific Rationale

**The question**: "Which specific cell types within my tissue drive the regulatory signals I see in bulk ENCODE data?"

ENCODE provides deeply sequenced bulk functional genomics (ChIP-seq, ATAC-seq, Hi-C) across hundreds of biosamples. But bulk data from a tissue like "pancreas" is a mixture of acinar cells (~80%), duct cells (~10%), endocrine cells (~5%), and others. An H3K27ac peak in bulk pancreas could be driven by any of these cell types. CellxGene Census provides cell-type-resolved expression data from 50M+ single-cell observations across thousands of datasets, enabling deconvolution of bulk ENCODE signals.

### The Bulk-to-Single-Cell Bridge

| Bulk ENCODE Signal | Single-Cell Question | CellxGene Answer |
|-------------------|---------------------|-----------------|
…

Read the whole file at its exact version.

How to install

Latest version
mdr add ammawla/encode-toolkit/cellxgene-context@git:20260321.5cfbcfd
Exact content
mdr add ammawla/encode-toolkit/cellxgene-context@sha256:3953cc0c269c1ada

Pin to a label to follow the author's releases, or to a sha256 to freeze the exact bytes forever. Either way the resolved hash is written to mdr.lock, and mdr install reproduces it on any machine.

Badge

mdr badge

[![mdr](https://markdownregistry.com/badge/art_5giv7pdx3ucotgf4.svg)](https://markdownregistry.com/a/art_5giv7pdx3ucotgf4)

1 badge views in 30 days

Versions

versioncommittedcommitsizeaudit
git:20260321.5cfbcfd latest2026-03-21 5cfbcfd 19,378 BA view · diff
git:20260312.665e7312026-03-12 665e731 19,378 BA view

Audit of the latest version

A  17 of 17 checks passed. Deterministic, no model, same answer every run.
  • pass: Frontmatter block present
  • pass: Frontmatter declares a name
  • pass: Frontmatter declares a description
  • pass: Size between 200 bytes and 200 KB (19378 bytes)
  • pass: No zero-width or bidi control characters
  • pass: No instruction hidden inside an HTML comment
  • pass: No link to an exfiltration or paste host
  • pass: No credential-shaped string
  • pass: No instruction to send local credentials anywhere
  • pass: No text hidden with inline styles
  • pass: No prompt-injection phrasing
  • pass: No curl or wget piped into a shell
  • pass: No recursive delete of root, home or parent
  • pass: No instruction to read or print local credentials
  • pass: No base64 blob over 200 characters
  • pass: No link to a raw IP address
  • pass: No script tag

Source

GitHub

ammawla/encode-toolkit · 20 stars · license AGPL-3.0 · pushed 2026-09-21 · branch main

API

GET https://markdownregistry.com/api/v1/artifacts/art_5giv7pdx3ucotgf4
GET https://markdownregistry.com/api/v1/resolve?ref=ammawla/encode-toolkit/cellxgene-context
GET https://markdownregistry.com/api/v1/blob/3953cc0c269c1ada061e7c7f3eb949347e0e4f0cf8c997fa790a62e4e2c4815a

Your agent does the legwork. You hear about the deals worth your word. Hand yours the standing instructions at modelranch.com and it joins the network that reads files like this one.

More from ammawla/encode-toolkit

accessibility-aggregation skill
ammawla/encode-toolkit · plugin/skills/accessibility-aggregation/SKILL.md · Build comprehensive chromatin accessibility maps by aggregating ATAC-seq and DNase-seq narrowPeak data across multiple…
git:20260312.665e731 · audit A · 20 stars
batch-analysis skill
ammawla/encode-toolkit · plugin/skills/batch-analysis/SKILL.md · Guide for multi-experiment batch operations: QC screening, batch download, comparison, and report generation across…
git:20260312.665e731 · audit A · 20 stars
bioinformatics-installer skill
ammawla/encode-toolkit · plugin/skills/bioinformatics-installer/SKILL.md · Install bioinformatics tools for ENCODE data analysis. Covers CLI tools (BWA, STAR, samtools, MACS2), R/Bioconductor…
git:20260321.5cfbcfd · audit B · 20 stars
cite-encode skill
ammawla/encode-toolkit · plugin/skills/cite-encode/SKILL.md · Generate proper ENCODE citations for publications, grants, and presentations. Use when the user needs to cite ENCODE…
git:20260312.665e731 · audit A · 20 stars
clinvar-annotation skill
ammawla/encode-toolkit · plugin/skills/clinvar-annotation/SKILL.md · Guide for annotating ENCODE regulatory variants with ClinVar clinical significance. Use when users need to check if…
git:20260312.665e731 · audit A · 20 stars
compare-biosamples skill
ammawla/encode-toolkit · plugin/skills/compare-biosamples/SKILL.md · Compare ENCODE experiments across different biosamples, tissues, or cell lines to identify tissue-specific regulatory…
git:20260312.665e731 · audit A · 20 stars
cross-reference skill
ammawla/encode-toolkit · plugin/skills/cross-reference/SKILL.md · Cross-reference ENCODE data with PubMed, bioRxiv, ClinicalTrials.gov, Open Targets, GTEx, ClinVar, GWAS Catalog…
git:20260312.665e731 · audit A · 20 stars
data-provenance skill
ammawla/encode-toolkit · plugin/skills/data-provenance/SKILL.md · Track exact provenance for every operation on ENCODE data — tool versions, reference files, scripts, parameters, and…
git:20260312.665e731 · audit A · 20 stars
disease-research skill
ammawla/encode-toolkit · plugin/skills/disease-research/SKILL.md · Use ENCODE functional genomics data for disease mechanism research. Use when the user wants to connect GWAS variants to…
git:20260321.5cfbcfd · audit A · 20 stars
download-encode skill
ammawla/encode-toolkit · plugin/skills/download-encode/SKILL.md · Download ENCODE genomics files (BED, FASTQ, BAM, bigWig, etc.) to the user's machine. Use when the user wants to…
git:20260321.5cfbcfd · audit A · 20 stars
ensembl-annotation skill
ammawla/encode-toolkit · plugin/skills/ensembl-annotation/SKILL.md · Query the Ensembl REST API for regulatory feature annotations, variant effect prediction (VEP), coordinate liftover…
git:20260312.665e731 · audit A · 20 stars
epigenome-profiling skill
ammawla/encode-toolkit · plugin/skills/epigenome-profiling/SKILL.md · Build comprehensive epigenomic profiles for tissues or cell types using ENCODE data. Use when the user wants to…
git:20260321.5cfbcfd · audit A · 20 stars

Every file in ammawla/encode-toolkit

Other files named cellxgene-context

cellxgene-context skill
ammawla/encode-toolkit · skills/cellxgene-context/SKILL.md · Guide for integrating CellxGene Census single-cell data with ENCODE bulk experiments. Use when users need…
git:20260920.6050003 · audit A · 20 stars

Browse by kind, by grade A, or by owner.