opentrons-thermocycler SKILL.md skillA
opentrons-thermocycler from jkitchin/knowhow is an agent skill: a SKILL.md folder an AI agent loads when a task calls for it.
mdr add jkitchin/knowhow/opentrons-thermocycler@git:20260307.3e74b2fPins git:20260307.3e74b2f in mdr.lock. Pin the fingerprint instead.
- Audit grade
- A, 17 of 17 checks passed
- Kind
- SKILL.md skill
- Repository
- jkitchin/knowhow
- Path
skills/laboratory/opentrons-thermocycler/SKILL.md- Versions recorded
- 2
- Latest version
- git:20260307.3e74b2f, committed Mar 7, 2026
- First version recorded
- Nov 29, 2025
- Size
- 19,713 bytes
- Repository stars
- 42
- License
- MIT
Opentrons Thermocycler Module - automated PCR thermal cycling with independent block (4-99°C) and lid (37-110°C) temperature control, profile execution, and auto-sealing lid support (GEN2) for high-throughput molecular biology workflows.
Collected from public GitHub. Every version is saved exactly as it was and never changes. Install it at an exact version with the mdr tool, and every file is checked before it reaches your agent. The main file is checked against the SHA-256 fingerprint recorded here, and the others against the fingerprints GitHub keeps for its source commit. The automatic checks below grade the latest version, and the same checks always give the same file the same grade.
What the file says
# Opentrons Thermocycler Module ## Overview The **Opentrons Thermocycler Module** automates PCR thermal cycling with precise, independent control of block temperature (4-99°C) and heated lid (37-110°C). Execute complex temperature profiles with automatic cycling, integrate with liquid handling for complete PCR setup automation, and use auto-sealing lids (GEN2) for walk-away workflows. **Core value:** Fully automate PCR setup and cycling. Load samples, dispense reagents, seal plates (GEN2), run thermal profiles, and collect products—all without manual intervention. ## When to Use Use the Thermocycler skill when: - Running PCR amplification protocols - Performing qPCR or RT-PCR reactions - Automating DNA/RNA thermal cycling workflows - Executing multi-step temperature incubations - Integrating thermal cycling with automated liquid handling - Setting up high-throughput PCR screening **Don't use when:** - Simple temperature control needed (use Temperature Module for 4-95°C range) - Shaking/mixing required during incubation (use Heater-Shaker Module) - Temperature outside 4-99°C range (block) or 37-110°C (lid) ## Quick Reference | Operation | Method | Key Parameters | …
Read the whole file at its exact version.
How to install
mdr add jkitchin/knowhow/opentrons-thermocycler@sha256:fdbcb142bbc9e885The line at the top of this page pins the label git:20260307.3e74b2f, which is what most people want. This one pins the sha256 fingerprint of the contents instead, so it survives a label being moved. Either way the exact version is written to mdr.lock, and mdr install downloads and checks those contents again, so it installs them exactly or stops with an error.
[](https://markdownregistry.com/a/art_6cvtbf2fclx4adht)
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Versions
The registry has recorded 2 versions of this file, between Nov 29, 2025 and Mar 7, 2026, about one every 98 days.
| version | committed | commit | size | audit | |
|---|---|---|---|---|---|
| git:20260307.3e74b2f latest | 2026-03-07 | 3e74b2f | 19,713 B | A | view · diff |
| git:20251129.b0f1718 | 2025-11-29 | b0f1718 | 19,515 B | A | view |
Audit of the latest version
- pass: Frontmatter block present
- pass: Frontmatter declares a name
- pass: Frontmatter declares a description
- pass: Size between 200 bytes and 200 KB (19713 bytes)
- pass: No zero-width or bidi control characters
- pass: No instruction hidden inside an HTML comment
- pass: No link to an exfiltration or paste host
- pass: No credential-shaped string
- pass: No instruction to send local credentials anywhere
- pass: No text hidden with inline styles
- pass: No prompt-injection phrasing
- pass: No curl or wget piped into a shell
- pass: No recursive delete of root, home or parent
- pass: No instruction to read or print local credentials
- pass: No base64 blob over 200 characters
- pass: No link to a raw IP address
- pass: No script tag
Source
jkitchin/knowhow · 42 stars · license MIT · pushed 2026-11-02 · branch main
API
GET https://markdownregistry.com/api/v1/artifacts/art_6cvtbf2fclx4adht GET https://markdownregistry.com/api/v1/resolve?ref=jkitchin/knowhow/opentrons-thermocycler GET https://markdownregistry.com/api/v1/blob/fdbcb142bbc9e885ce8258091b83a97ae2e2392b6f1b65ca2488483abc0b0435
Your AI assistant does the legwork. You hear about the deals worth your word. Hand yours the standing instructions at modelranch.com and it joins the network that reads files like this one.
Guides: the SKILL.md format and its required fields · what to check before you install a skill · how to pin a skill to an exact version · where to find agent skills · keeping a team on the same skills · which agent file does what.