bio-genome-assembly-assembly-qc SKILL.md skillA
bio-genome-assembly-assembly-qc from fridrichmethod/awesome-skills is an agent skill: a SKILL.md folder an AI agent loads when a task calls for it.
mdr add fridrichmethod/awesome-skills/bio-genome-assembly-assembly-qc@git:20260531.6e617c4Pins git:20260531.6e617c4 in mdr.lock. Pin the fingerprint instead.
- Audit grade
- A, 17 of 17 checks passed
- Kind
- SKILL.md skill
- Repository
- fridrichmethod/awesome-skills
- Path
skills/assembly-qc/SKILL.md- Versions recorded
- 2
- Latest version
- git:20260531.6e617c4, committed May 31, 2026
- First version recorded
- May 17, 2026
- Size
- 19,594 bytes
- Repository stars
- 15
- License
- a license file GitHub does not identify
Evaluates genome assembly quality across the three orthogonal axes - contiguity (QUAST auN/NG50/NGx, not bare N50), completeness (BUSCO/compleasm gene-space plus Merqury k-mer completeness), and correctness (reference-free Merqury QV, Inspector/CRAQ structural errors, asmgene false-duplication/collapse). Covers why N50 is the most-gamed metric, why QV measured on the polishing reads is circular, distinguishing uncollapsed haplotigs from real WGD, and the EBP/VGP 6.C.Q40 standard. Use when…
Collected from public GitHub. Every version is saved exactly as it was and never changes. Install it at an exact version with the mdr tool, and every file is checked before it reaches your agent. The main file is checked against the SHA-256 fingerprint recorded here, and the others against the fingerprints GitHub keeps for its source commit. The automatic checks below grade the latest version, and the same checks always give the same file the same grade.
What the file says
## Version Compatibility Reference examples tested with: QUAST 5.2+, BUSCO 5.5+ (and 6.x for odb12 lineages), compleasm 0.2.6+, Merqury 1.3+, meryl 1.4+, minimap2 2.26+, Inspector 1.2+, CRAQ 1.0+, merfin 1.0+, GenomeScope2 2.0+. Before using code patterns, verify installed versions match. If versions differ: - CLI: `<tool> --version` then `<tool> --help` to confirm flags - Python: `pip show <package>` then `help(module.function)` to check signatures Results depend on inputs that outlive the binary version - record them: - BUSCO/compleasm depend on the **lineage dataset** and OrthoDB generation. `_odb10` (BUSCO 5) and `_odb12` (BUSCO 6 default) gene sets are not comparable across the version boundary; a 99% on the shallow `eukaryota_odb10` (~255 genes) is a different claim from 99% on a deep clade set (~5,500+). - Merqury QV/completeness depend on the **k-mer size** (from `best_k.sh <genome_size>`, not hardcoded) and the **read set** used for the k-mer DB (use accurate reads; see the circularity warning below). - NG50/NGx/auNG depend on the **expected genome-size estimate** (GenomeScope2 / flow cytometry / a congener). …
Read the whole file at its exact version.
How to install
mdr add fridrichmethod/awesome-skills/bio-genome-assembly-assembly-qc@sha256:b0d047e97518de28The line at the top of this page pins the label git:20260531.6e617c4, which is what most people want. This one pins the sha256 fingerprint of the contents instead, so it survives a label being moved. Either way the exact version is written to mdr.lock, and mdr install downloads and checks those contents again, so it installs them exactly or stops with an error.
[](https://markdownregistry.com/a/art_gfbdr2ddxql5u5bv)
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Versions
The registry has recorded 2 versions of this file, between May 17, 2026 and May 31, 2026, about one every 14 days.
| version | committed | commit | size | audit | |
|---|---|---|---|---|---|
| git:20260531.6e617c4 latest | 2026-05-31 | 6e617c4 | 19,594 B | A | view · diff |
| git:20260517.81511a7 | 2026-05-17 | 81511a7 | 8,767 B | A | view |
Audit of the latest version
- pass: Frontmatter block present
- pass: Frontmatter declares a name
- pass: Frontmatter declares a description
- pass: Size between 200 bytes and 200 KB (19594 bytes)
- pass: No zero-width or bidi control characters
- pass: No instruction hidden inside an HTML comment
- pass: No link to an exfiltration or paste host
- pass: No credential-shaped string
- pass: No instruction to send local credentials anywhere
- pass: No text hidden with inline styles
- pass: No prompt-injection phrasing
- pass: No curl or wget piped into a shell
- pass: No recursive delete of root, home or parent
- pass: No instruction to read or print local credentials
- pass: No base64 blob over 200 characters
- pass: No link to a raw IP address
- pass: No script tag
Source
fridrichmethod/awesome-skills · 15 stars · license a license file GitHub does not identify · pushed 2026-10-18 · branch main
API
GET https://markdownregistry.com/api/v1/artifacts/art_gfbdr2ddxql5u5bv GET https://markdownregistry.com/api/v1/resolve?ref=fridrichmethod/awesome-skills/bio-genome-assembly-assembly-qc GET https://markdownregistry.com/api/v1/blob/b0d047e97518de285a13efc73ffe6035739aa614cd1ac40aad3b47935628e178
Your AI assistant does the legwork. You hear about the deals worth your word. Hand yours the standing instructions at modelranch.com and it joins the network that reads files like this one.
Guides: the SKILL.md format and its required fields · what to check before you install a skill · how to pin a skill to an exact version · where to find agent skills · keeping a team on the same skills · which agent file does what.