opentrons-absorbance-reader SKILL.md skillA

opentrons-absorbance-reader from jkitchin/knowhow is an agent skill: a SKILL.md folder an AI agent loads when a task calls for it.

Install it, pinned
mdr add jkitchin/knowhow/opentrons-absorbance-reader@git:20260307.3e74b2f

Pins git:20260307.3e74b2f in mdr.lock. Pin the fingerprint instead.

Audit grade
A, 17 of 17 checks passed
Kind
SKILL.md skill
Repository
jkitchin/knowhow
Path
skills/laboratory/opentrons-absorbance-reader/SKILL.md
Versions recorded
2
Latest version
git:20260307.3e74b2f, committed Mar 7, 2026
First version recorded
Nov 29, 2025
Size
18,498 bytes
Repository stars
42
License
MIT

Opentrons Absorbance Plate Reader Module for Flex - on-deck microplate spectrophotometry with single/multi-wavelength reading (380-740nm in 10nm increments), automated lid control, and CSV data export for ELISA, cell growth, and colorimetric assays.

Collected from public GitHub. Every version is saved exactly as it was and never changes. Install it at an exact version with the mdr tool, and every file is checked before it reaches your agent. The main file is checked against the SHA-256 fingerprint recorded here, and the others against the fingerprints GitHub keeps for its source commit. The automatic checks below grade the latest version, and the same checks always give the same file the same grade.

What the file says

# Opentrons Absorbance Plate Reader Module

## Overview

The **Absorbance Plate Reader Module** is an on-deck microplate spectrophotometer exclusively for Opentrons Flex. It measures light absorbance in 96-well plates at up to 6 wavelengths simultaneously, enabling automated ELISA, cell density measurements, colorimetric assays, and kinetic readings without manual plate transfers.

**Core value:** Eliminate manual plate reader transfers. Read absorbance mid-protocol to make real-time decisions or collect kinetic data with automated timing and liquid handling.

**Platform:** Opentrons Flex only (not compatible with OT-2)

## When to Use

Use the Absorbance Reader skill when:
- Running ELISA or other colorimetric assays requiring absorbance reading
- Measuring bacterial or cell culture growth (OD600)
- Performing kinetic assays with timed readings
- Collecting multi-wavelength absorbance data
- Protocols requiring real-time decision-making based on absorbance values
- Automating plate-based biochemical assays

**Don't use when:**
- Working with OT-2 robot (module is Flex-only)
- Need wavelengths outside 380-740nm range
- Require fluorescence or luminescence measurements
…

Read the whole file at its exact version.

How to install

The exact contents, by fingerprint
mdr add jkitchin/knowhow/opentrons-absorbance-reader@sha256:5c34db10f4bb7662

The line at the top of this page pins the label git:20260307.3e74b2f, which is what most people want. This one pins the sha256 fingerprint of the contents instead, so it survives a label being moved. Either way the exact version is written to mdr.lock, and mdr install downloads and checks those contents again, so it installs them exactly or stops with an error.

Badge

mdr badge

[![mdr](https://markdownregistry.com/badge/art_rl7wsjn34ip25izl.svg)](https://markdownregistry.com/a/art_rl7wsjn34ip25izl)

0 badge image fetches in 30 days. What the badge shows.

Versions

The registry has recorded 2 versions of this file, between Nov 29, 2025 and Mar 7, 2026, about one every 98 days.

versioncommittedcommitsizeaudit
git:20260307.3e74b2f latest2026-03-07 3e74b2f 18,498 BA view · diff
git:20251129.b0f17182025-11-29 b0f1718 18,487 BA view

Audit of the latest version

A  17 of 17 checks passed. Fixed automatic checks with no AI involved, so the same checks give the same answer every run.
  • pass: Frontmatter block present
  • pass: Frontmatter declares a name
  • pass: Frontmatter declares a description
  • pass: Size between 200 bytes and 200 KB (18498 bytes)
  • pass: No zero-width or bidi control characters
  • pass: No instruction hidden inside an HTML comment
  • pass: No link to an exfiltration or paste host
  • pass: No credential-shaped string
  • pass: No instruction to send local credentials anywhere
  • pass: No text hidden with inline styles
  • pass: No prompt-injection phrasing
  • pass: No curl or wget piped into a shell
  • pass: No recursive delete of root, home or parent
  • pass: No instruction to read or print local credentials
  • pass: No base64 blob over 200 characters
  • pass: No link to a raw IP address
  • pass: No script tag

Source

GitHub

jkitchin/knowhow · 42 stars · license MIT · pushed 2026-11-02 · branch main

API

GET https://markdownregistry.com/api/v1/artifacts/art_rl7wsjn34ip25izl
GET https://markdownregistry.com/api/v1/resolve?ref=jkitchin/knowhow/opentrons-absorbance-reader
GET https://markdownregistry.com/api/v1/blob/5c34db10f4bb76622c73f0f9313c9a493bd09c397f7256464541eeb84ab1201e

Your AI assistant does the legwork. You hear about the deals worth your word. Hand yours the standing instructions at modelranch.com and it joins the network that reads files like this one.

Guides: the SKILL.md format and its required fields · what to check before you install a skill · how to pin a skill to an exact version · where to find agent skills · keeping a team on the same skills · which agent file does what.

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