bio-analytical-validation SKILL.md skillA
bio-analytical-validation from fridrichmethod/awesome-skills is an agent skill: a SKILL.md folder an AI agent loads when a task calls for it.
mdr add fridrichmethod/awesome-skills/bio-analytical-validation@git:20260607.d890519Pins git:20260607.d890519 in mdr.lock. Pin the fingerprint instead.
- Audit grade
- A, 17 of 17 checks passed
- Kind
- SKILL.md skill
- Repository
- fridrichmethod/awesome-skills
- Path
skills/analytical-validation/SKILL.md- Versions recorded
- 1
- Latest version
- git:20260607.d890519, committed Jun 7, 2026
- Size
- 16,709 bytes
- Repository stars
- 15
- License
- a license file GitHub does not identify
Treats a ctDNA assay as a molecule-counting experiment at the Poisson edge and builds its analytical-validation case the measurement-science way. Covers the genome-equivalent currency (~330 haploid copies/ng), the lambda = input_GE x VAF sampling ceiling (lambda>=3 for ~95% detection), the error-suppression ladder (raw NGS ~1e-3 -> single-strand UMI ~1e-4/1e-5 -> duplex <1e-7), the CLSI EP17 LoB/LoD/LoD95/LoQ framework, the per-locus-vs-panel-integrated LoD distinction that lets bespoke MRD…
Collected from public GitHub. Every version is saved exactly as it was and never changes. Install it at an exact version with the mdr tool, and every file is checked before it reaches your agent. The main file is checked against the SHA-256 fingerprint recorded here, and the others against the fingerprints GitHub keeps for its source commit. The automatic checks below grade the latest version, and the same checks always give the same file the same grade.
What the file says
## Version Compatibility Reference examples tested with: numpy 1.26+, scipy 1.12+, statsmodels 0.14+ Before using code patterns, verify installed versions match. If versions differ: - Python: `pip show <package>` then `help(module.function)` to check signatures If code throws ImportError, AttributeError, or TypeError, introspect the installed package and adapt the example to match the actual API rather than retrying. # Analytical Validation and Detection Limits **"What is the real limit of detection of my ctDNA assay, and can I trust the number I am about to report?"** -> Quantify the Poisson sampling ceiling, the error-suppression floor, and the LoB/LoD/LoQ that together define a defensible sensitivity claim. - Python: `scipy.stats.poisson` for detection-probability math, `scipy.stats.norm` for CLSI LoB/LoD, `statsmodels` Probit/Logit for a dilution-series LoD95 fit. ## The Single Most Important Modern Insight -- LoD Is Set by Genome Equivalents Sampled and Error Suppression, NOT by Sequencing Depth or the Caller …
Read the whole file at its exact version.
How to install
mdr add fridrichmethod/awesome-skills/bio-analytical-validation@sha256:11ebcaf5d0a7e471The line at the top of this page pins the label git:20260607.d890519, which is what most people want. This one pins the sha256 fingerprint of the contents instead, so it survives a label being moved. Either way the exact version is written to mdr.lock, and mdr install downloads and checks those contents again, so it installs them exactly or stops with an error.
[](https://markdownregistry.com/a/art_wvl5g7236l235per)
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Versions
The registry has recorded 1 version of this file, committed on Jun 7, 2026.
Audit of the latest version
- pass: Frontmatter block present
- pass: Frontmatter declares a name
- pass: Frontmatter declares a description
- pass: Size between 200 bytes and 200 KB (16709 bytes)
- pass: No zero-width or bidi control characters
- pass: No instruction hidden inside an HTML comment
- pass: No link to an exfiltration or paste host
- pass: No credential-shaped string
- pass: No instruction to send local credentials anywhere
- pass: No text hidden with inline styles
- pass: No prompt-injection phrasing
- pass: No curl or wget piped into a shell
- pass: No recursive delete of root, home or parent
- pass: No instruction to read or print local credentials
- pass: No base64 blob over 200 characters
- pass: No link to a raw IP address
- pass: No script tag
Source
fridrichmethod/awesome-skills · 15 stars · license a license file GitHub does not identify · pushed 2026-10-18 · branch main
API
GET https://markdownregistry.com/api/v1/artifacts/art_wvl5g7236l235per GET https://markdownregistry.com/api/v1/resolve?ref=fridrichmethod/awesome-skills/bio-analytical-validation GET https://markdownregistry.com/api/v1/blob/11ebcaf5d0a7e471f95ec29ea477b55614a44725bc5840c4cb6da8dc78d41f7b
Your AI assistant does the legwork. You hear about the deals worth your word. Hand yours the standing instructions at modelranch.com and it joins the network that reads files like this one.
Guides: the SKILL.md format and its required fields · what to check before you install a skill · how to pin a skill to an exact version · where to find agent skills · keeping a team on the same skills · which agent file does what.