bio-bam-statistics SKILL.md skillA

bio-bam-statistics from fridrichmethod/awesome-skills is an agent skill: a SKILL.md folder an AI agent loads when a task calls for it.

Install it, pinned
mdr add fridrichmethod/awesome-skills/bio-bam-statistics@git:20260712.2ff971b

Pins git:20260712.2ff971b in mdr.lock. Pin the fingerprint instead.

Audit grade
A, 17 of 17 checks passed
Kind
SKILL.md skill
Repository
fridrichmethod/awesome-skills
Path
skills/bam-statistics/SKILL.md
Versions recorded
4
Latest version
git:20260712.2ff971b, committed Jul 12, 2026
First version recorded
May 17, 2026
Size
15,465 bytes
Repository stars
15
License
a license file GitHub does not identify

Generate alignment statistics using samtools flagstat, stats, depth, coverage, and mosdepth. Use when assessing alignment quality, calculating coverage, or generating QC reports.

Collected from public GitHub. Every version is saved exactly as it was and never changes. Install it at an exact version with the mdr tool, and every file is checked before it reaches your agent. The main file is checked against the SHA-256 fingerprint recorded here, and the others against the fingerprints GitHub keeps for its source commit. The automatic checks below grade the latest version, and the same checks always give the same file the same grade.

What the file says

## Version Compatibility

Reference examples tested with: pysam 0.22+, samtools 1.19+

Before using code patterns, verify installed versions match. If versions differ:
- Python: `pip show <package>` then `help(module.function)` to check signatures
- CLI: `<tool> --version` then `<tool> --help` to confirm flags

If code throws ImportError, AttributeError, or TypeError, introspect the installed
package and adapt the example to match the actual API rather than retrying.

# BAM Statistics

**"Get alignment statistics and coverage from my BAM file"** -> Generate read counts, mapping rates, per-chromosome statistics, depth profiles, and coverage summaries.
- CLI: `samtools flagstat`, `samtools stats`, `samtools depth`, `samtools coverage` (samtools)
- Python: `pysam.AlignmentFile` with `pileup()` and `get_index_statistics()` (pysam)

Generate alignment statistics using samtools and pysam.

## Quick Summary Commands

| Question | Best tool | Why |
|----------|-----------|-----|
| Quick read counts by FLAG category | `samtools flagstat` | Fast; counts secondary+supp in totals |
| Per-chromosome counts | `samtools idxstats` | Fast (needs index); **counts secondary+supp** |
…

Read the whole file at its exact version.

How to install

The exact contents, by fingerprint
mdr add fridrichmethod/awesome-skills/bio-bam-statistics@sha256:fb86e671b6386f84

The line at the top of this page pins the label git:20260712.2ff971b, which is what most people want. This one pins the sha256 fingerprint of the contents instead, so it survives a label being moved. Either way the exact version is written to mdr.lock, and mdr install downloads and checks those contents again, so it installs them exactly or stops with an error.

Badge

mdr badge

[![mdr](https://markdownregistry.com/badge/art_y33ry4fuyddyrw5f.svg)](https://markdownregistry.com/a/art_y33ry4fuyddyrw5f)

3 badge image fetches in 30 days. What the badge shows.

Versions

The registry has recorded 4 versions of this file, between May 17, 2026 and Jul 12, 2026, about one every 19 days.

versioncommittedcommitsizeaudit
git:20260712.2ff971b latest2026-07-12 2ff971b 15,465 BA view · diff
git:20260531.6e617c42026-05-31 6e617c4 15,090 BA view · diff
git:20260528.89ddef62026-05-28 89ddef6 15,091 BA view · diff
git:20260517.81511a72026-05-17 81511a7 14,839 BA view

Audit of the latest version

A  17 of 17 checks passed. Fixed automatic checks with no AI involved, so the same checks give the same answer every run.
  • pass: Frontmatter block present
  • pass: Frontmatter declares a name
  • pass: Frontmatter declares a description
  • pass: Size between 200 bytes and 200 KB (15465 bytes)
  • pass: No zero-width or bidi control characters
  • pass: No instruction hidden inside an HTML comment
  • pass: No link to an exfiltration or paste host
  • pass: No credential-shaped string
  • pass: No instruction to send local credentials anywhere
  • pass: No text hidden with inline styles
  • pass: No prompt-injection phrasing
  • pass: No curl or wget piped into a shell
  • pass: No recursive delete of root, home or parent
  • pass: No instruction to read or print local credentials
  • pass: No base64 blob over 200 characters
  • pass: No link to a raw IP address
  • pass: No script tag

Source

GitHub

fridrichmethod/awesome-skills · 15 stars · license a license file GitHub does not identify · pushed 2026-11-02 · branch main

API

GET https://markdownregistry.com/api/v1/artifacts/art_y33ry4fuyddyrw5f
GET https://markdownregistry.com/api/v1/resolve?ref=fridrichmethod/awesome-skills/bio-bam-statistics
GET https://markdownregistry.com/api/v1/blob/fb86e671b6386f84ca090ce941b0f71d5baddc50b4dbb346a456b47595fd2301

Your AI assistant does the legwork. You hear about the deals worth your word. Hand yours the standing instructions at modelranch.com and it joins the network that reads files like this one.

Guides: the SKILL.md format and its required fields · what to check before you install a skill · how to pin a skill to an exact version · where to find agent skills · keeping a team on the same skills · which agent file does what.

More from fridrichmethod/awesome-skills

13c-metabolic-flux SKILL.md skill
fridrichmethod/awesome-skills · skills/13c-metabolic-flux/SKILL.md · Estimates intracellular metabolic fluxes from steady-state carbon-13 isotope-tracing measurements using validated atom…
v1.2 · audit A · 15 stars
aav-vector-design-agent SKILL.md skill
fridrichmethod/awesome-skills · skills/aav-vector-design-agent/SKILL.md · AI-powered adeno-associated virus (AAV) vector design for gene therapy including capsid engineering, promoter…
git:20260520.7a7a671 · audit A · 15 stars
bio-metagenomics-abundance SKILL.md skill
fridrichmethod/awesome-skills · skills/abundance-estimation/SKILL.md · Turns shotgun classifier output into a defensible abundance table with Bracken Bayesian re-estimation, then…
git:20260614.5d4591c · audit A · 15 stars
academic-humanizer SKILL.md skill
fridrichmethod/awesome-skills · skills/academic-humanizer/SKILL.md · "Improve the clarity and voice of AI-assisted academic writing (papers, theses, rebuttals) and
v0.3.3 · audit A · 15 stars
academic-paper SKILL.md skill
fridrichmethod/awesome-skills · skills/academic-paper--imbad-academic--73667df0a3/SKILL.md · 12-agent academic paper writing pipeline. 11 modes (full/plan/outline/revision/revision-coach/abstract/lit-review/format…
v3.3.1 · audit A · 15 stars
academic-paper-reviewer SKILL.md skill
fridrichmethod/awesome-skills · skills/academic-paper-reviewer--imbad-academic--277487101b/SKILL.md · Multi-perspective academic paper review with dynamic reviewer personas. Runs a 5-seat, role-separated review panel…
v1.11.1 · audit A · 15 stars
academic-paper-reviewer SKILL.md skill
fridrichmethod/awesome-skills · skills/academic-paper-reviewer/SKILL.md · Multi-perspective academic paper review with dynamic reviewer personas. Runs a 5-seat, role-separated review panel…
v1.11.1 · audit A · 15 stars
academic-paper SKILL.md skill
fridrichmethod/awesome-skills · skills/academic-paper/SKILL.md · 12-agent academic paper writing pipeline. 11 modes (full/plan/outline/revision/revision-coach/abstract/lit-review/format…
v3.3.1 · audit A · 15 stars
academic-pipeline SKILL.md skill
fridrichmethod/awesome-skills · skills/academic-pipeline--imbad-academic--cbc2ab73c3/SKILL.md · Orchestrator for the full academic research pipeline: research -> write -> integrity check -> review -> revise ->…
v3.24.0 · audit A · 15 stars
academic-pipeline SKILL.md skill
fridrichmethod/awesome-skills · skills/academic-pipeline/SKILL.md · Orchestrator for the full academic research pipeline: research -> write -> integrity check -> review -> revise ->…
v3.23.0 · audit A · 15 stars
academic-writing SKILL.md skill
fridrichmethod/awesome-skills · skills/academic-writing--journalism--0c1ed18632/SKILL.md · Scholarly writing and research compliance. Use for CRediT, preregistration, Plan S, Nelson Memo, preprints, ORCID, LLM…
git:20261025.50a54c2 · audit A · 15 stars
academic-writing SKILL.md skill
fridrichmethod/awesome-skills · skills/academic-writing/SKILL.md · Scholarly writing and research compliance. Use for CRediT, preregistration, Plan S, Nelson Memo, preprints, ORCID, LLM…
git:20260823.528ff10 · audit A · 15 stars

Every file in fridrichmethod/awesome-skills

Other files named bio-bam-statistics

bio-bam-statistics SKILL.md skill
pku-yuangroup/openai4s · skills/bioskills/bio-alignment-files-bam-statistics/SKILL.md · Generate alignment statistics using samtools flagstat, stats, depth, coverage, and mosdepth. Use when assessing…
git:20260821.2d1b678 · audit A · 662 stars

Browse by kind, by grade A, or by owner.