Home / ammawla / encode-toolkit · plugin/skills/scrna-meta-analysis/SKILL.md · GitHub

scrna-meta-analysis skillA

scrna-meta-analysis is agent-read markdown (skill) from ammawla/encode-toolkit: Conduct rigorous cross-study meta-analysis of scRNA-seq data from ENCODE, integrating multiple single-cell transcriptomic datasets for a tissue/cell type. Use when the user wants to answer "what cell types exist in my tissue and what genes define them?" by combining scRNA-seq data across donors, labs, and platforms. Follows the Mawla et al. 2019 framework for assessing cross-study reproducibility, TIN-based quality filtering, and detection-limit-aware interpretation. Handles batch correction (Ha.

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What the file says

# Cross-Study Meta-Analysis of scRNA-seq Data

## When to Use

- User wants to perform meta-analysis across multiple single-cell RNA-seq datasets
- User asks about "scRNA-seq meta-analysis", "dataset integration", "batch correction", or "cross-study comparison"
- User needs to harmonize cell type annotations across studies from different labs
- User wants to build reference atlases or identify conserved cell populations across datasets
- Example queries: "integrate 5 scRNA-seq datasets from different labs", "harmonize cell type labels across studies", "meta-analyze single-cell data for pancreas"

Integrate multiple ENCODE scRNA-seq datasets for a tissue/cell type into a unified cell atlas with reproducibility-aware quality assessment.

## Scientific Rationale

**The question**: "What cell types and transcriptional programs are present in my tissue, and which findings are reproducible across studies?"
…

Read the whole file at its exact version.

How to install

Latest version
mdr add ammawla/encode-toolkit/scrna-meta-analysis@git:20260920.6050003
Exact content
mdr add ammawla/encode-toolkit/scrna-meta-analysis@sha256:00853f93de97ae69

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Versions

versioncommittedcommitsizeaudit
git:20260920.6050003 latest2026-09-20 6050003 31,084 BA view · diff
git:20260920.ce7840d2026-09-20 ce7840d 30,250 BA view · diff
git:20260321.d9029d02026-03-21 d9029d0 30,262 BA view · diff
git:20260312.be327062026-03-12 be32706 30,311 BA view

Audit of the latest version

A  17 of 17 checks passed. Deterministic, no model, same answer every run.
  • pass: Frontmatter block present
  • pass: Frontmatter declares a name
  • pass: Frontmatter declares a description
  • pass: Size between 200 bytes and 200 KB (31084 bytes)
  • pass: No zero-width or bidi control characters
  • pass: No instruction hidden inside an HTML comment
  • pass: No link to an exfiltration or paste host
  • pass: No credential-shaped string
  • pass: No instruction to send local credentials anywhere
  • pass: No text hidden with inline styles
  • pass: No prompt-injection phrasing
  • pass: No curl or wget piped into a shell
  • pass: No recursive delete of root, home or parent
  • pass: No instruction to read or print local credentials
  • pass: No base64 blob over 200 characters
  • pass: No link to a raw IP address
  • pass: No script tag

Source

GitHub

ammawla/encode-toolkit · 20 stars · license AGPL-3.0 · pushed 2026-09-21 · branch main

API

GET https://markdownregistry.com/api/v1/artifacts/art_bzkmz4xfx7nhwazz
GET https://markdownregistry.com/api/v1/resolve?ref=ammawla/encode-toolkit/scrna-meta-analysis
GET https://markdownregistry.com/api/v1/blob/00853f93de97ae69b69ecb7f71522b804740e81628a203ff504224f39ff0daa3

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