star-rna-seq-aligner SKILL.md skillA

star-rna-seq-aligner from jaechang-hits/sciagent-skills is an agent skill: a SKILL.md folder an AI agent loads when a task calls for it.

Install it, pinned
mdr add jaechang-hits/sciagent-skills/star-rna-seq-aligner@git:20260929.7a7d60f

Pins git:20260929.7a7d60f in mdr.lock. Pin the fingerprint instead.

Audit grade
A, 17 of 17 checks passed
Kind
SKILL.md skill
Repository
jaechang-hits/sciagent-skills
Path
skills/genomics-bioinformatics/alignment/star-rna-seq-aligner/SKILL.md
Versions recorded
3
Latest version
git:20260929.7a7d60f, committed Sep 29, 2026
First version recorded
May 28, 2026
Size
16,440 bytes
Repository stars
381
License
a license file GitHub does not identify

Splice-aware RNA-seq aligner producing sorted BAM and splice junction tables. Builds genome index, runs two-pass alignment for better junctions. Outputs sorted BAM, junctions (SJ.out.tab), stats (Log.final.out), optional gene counts. Use Salmon for fast pseudoalignment; STAR when a BAM is needed for variant calling, IGV, or ENCODE pipelines.

Collected from public GitHub. Every version is saved exactly as it was and never changes. Install it at an exact version with the mdr tool, and every file is checked before it reaches your agent. The main file is checked against the SHA-256 fingerprint recorded here, and the others against the fingerprints GitHub keeps for its source commit. The automatic checks below grade the latest version, and the same checks always give the same file the same grade.

What the file says

# STAR — Spliced RNA-seq Aligner

## Overview

STAR (Spliced Transcripts Alignment to a Reference) aligns RNA-seq reads to a genome in a splice-aware manner, identifying novel and annotated splice junctions in a single pass. It generates coordinate-sorted BAM files compatible with samtools, IGV, deeptools, and GATK. STAR's 2-pass mode re-aligns reads using junctions discovered in the first pass, improving sensitivity for novel splice sites. With `--quantMode GeneCounts`, STAR simultaneously produces gene-level read count tables without requiring a separate featureCounts or HTSeq step.

## When to Use

- Aligning bulk RNA-seq reads to a reference genome when downstream tools require a BAM file (variant calling, visualization, deeptools)
- Running ENCODE-compliant RNA-seq pipelines that mandate genome alignment
- Discovering novel splice junctions and alternative splicing events in the dataset
- Generating gene count tables alongside BAM alignment in a single step with `--quantMode GeneCounts`
- Processing long reads or reads with high mismatch rates by tuning `--outFilterMismatchNmax`
…

Read the whole file at its exact version.

How to install

The exact contents, by fingerprint
mdr add jaechang-hits/sciagent-skills/star-rna-seq-aligner@sha256:bfd5bfbcc5b31551

The line at the top of this page pins the label git:20260929.7a7d60f, which is what most people want. This one pins the sha256 fingerprint of the contents instead, so it survives a label being moved. Either way the exact version is written to mdr.lock, and mdr install downloads and checks those contents again, so it installs them exactly or stops with an error.

Badge

mdr badge

[![mdr](https://markdownregistry.com/badge/art_hfwajsmw64s5bm4j.svg)](https://markdownregistry.com/a/art_hfwajsmw64s5bm4j)

4 badge image fetches in 30 days. What the badge shows.

Versions

The registry has recorded 3 versions of this file, between May 28, 2026 and Sep 29, 2026, about one every 62 days.

versioncommittedcommitsizeaudit
git:20260929.7a7d60f latest2026-09-29 7a7d60f 16,440 BA view · diff
git:20260929.35fe6e32026-09-29 35fe6e3 16,489 BA view · diff
git:20260528.3e3f9982026-05-28 3e3f998 14,096 BA view

Audit of the latest version

A  17 of 17 checks passed. Fixed automatic checks with no AI involved, so the same checks give the same answer every run.
  • pass: Frontmatter block present
  • pass: Frontmatter declares a name
  • pass: Frontmatter declares a description
  • pass: Size between 200 bytes and 200 KB (16440 bytes)
  • pass: No zero-width or bidi control characters
  • pass: No instruction hidden inside an HTML comment
  • pass: No link to an exfiltration or paste host
  • pass: No credential-shaped string
  • pass: No instruction to send local credentials anywhere
  • pass: No text hidden with inline styles
  • pass: No prompt-injection phrasing
  • pass: No curl or wget piped into a shell
  • pass: No recursive delete of root, home or parent
  • pass: No instruction to read or print local credentials
  • pass: No base64 blob over 200 characters
  • pass: No link to a raw IP address
  • pass: No script tag

Source

GitHub

jaechang-hits/sciagent-skills · 381 stars · license a license file GitHub does not identify · pushed 2026-10-16 · branch main

API

GET https://markdownregistry.com/api/v1/artifacts/art_hfwajsmw64s5bm4j
GET https://markdownregistry.com/api/v1/resolve?ref=jaechang-hits/sciagent-skills/star-rna-seq-aligner
GET https://markdownregistry.com/api/v1/blob/bfd5bfbcc5b3155122f843190d4cb6cb11d835b25ebce704cb5940abad5a4b61

Your AI assistant does the legwork. You hear about the deals worth your word. Hand yours the standing instructions at modelranch.com and it joins the network that reads files like this one.

Guides: the SKILL.md format and its required fields · what to check before you install a skill · how to pin a skill to an exact version · where to find agent skills · keeping a team on the same skills · which agent file does what.

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