Home / ammawla / encode-toolkit · plugin/skills/regulatory-elements/SKILL.md · GitHub

regulatory-elements skillA

regulatory-elements is agent-read markdown (skill) from ammawla/encode-toolkit: Discover and characterize regulatory elements (enhancers, promoters, silencers, insulators, super-enhancers) using ENCODE data and the cCRE catalog. Use when the user wants to find candidate regulatory elements, identify active enhancers in a tissue, map promoter states, classify chromatin states with ChromHMM, identify super-enhancers with ROSE, understand the functional validation hierarchy (CRISPR > MPRA > reporter), or characterize non-coding genomic regions. Covers the full workflow from EN.

Indexed from public GitHub and served as immutable, content-addressed versions. Install it pinned to an exact SHA-256 with the mdr CLI, and every file is verified against the hash recorded here before it reaches your agent. The deterministic audit below grades the latest version, and the same file always earns the same grade.

What the file says

# Discover and Characterize Regulatory Elements with ENCODE

## When to Use

- User wants to find enhancers, promoters, silencers, or insulators in a specific tissue using ENCODE
- User asks about "regulatory elements", "cCREs", "enhancer discovery", "ChromHMM", or "super-enhancers"
- User needs to classify chromatin states or identify active regulatory regions from histone mark data
- User wants to use ENCODE's 926,535 cCRE catalog or run functional validation (CRISPR/MPRA/reporter)
- Example queries: "find active enhancers in liver", "classify chromatin states for my tissue", "identify super-enhancers from H3K27ac data"

Identify, classify, and functionally characterize regulatory elements using ENCODE's catalog of 926,535 human candidate cis-regulatory elements (cCREs) and layered functional genomics data.

## Scientific Rationale

**The question**: "What regulatory elements are active in my tissue of interest, and what are they doing?"
…

Read the whole file at its exact version.

How to install

Latest version
mdr add ammawla/encode-toolkit/regulatory-elements@git:20260920.6050003
Exact content
mdr add ammawla/encode-toolkit/regulatory-elements@sha256:5d1993bfd3bfdb64

Pin to a label to follow the author's releases, or to a sha256 to freeze the exact bytes forever. Either way the resolved hash is written to mdr.lock, and mdr install reproduces it on any machine.

Badge

mdr badge

[![mdr](https://markdownregistry.com/badge/art_jrixkuxpvigfy3h7.svg)](https://markdownregistry.com/a/art_jrixkuxpvigfy3h7)

1 badge views in 30 days

Versions

versioncommittedcommitsizeaudit
git:20260920.6050003 latest2026-09-20 6050003 27,702 BA view · diff
git:20260920.ce7840d2026-09-20 ce7840d 27,114 BA view · diff
git:20260312.be327062026-03-12 be32706 27,126 BA view

Audit of the latest version

A  17 of 17 checks passed. Deterministic, no model, same answer every run.
  • pass: Frontmatter block present
  • pass: Frontmatter declares a name
  • pass: Frontmatter declares a description
  • pass: Size between 200 bytes and 200 KB (27702 bytes)
  • pass: No zero-width or bidi control characters
  • pass: No instruction hidden inside an HTML comment
  • pass: No link to an exfiltration or paste host
  • pass: No credential-shaped string
  • pass: No instruction to send local credentials anywhere
  • pass: No text hidden with inline styles
  • pass: No prompt-injection phrasing
  • pass: No curl or wget piped into a shell
  • pass: No recursive delete of root, home or parent
  • pass: No instruction to read or print local credentials
  • pass: No base64 blob over 200 characters
  • pass: No link to a raw IP address
  • pass: No script tag

Source

GitHub

ammawla/encode-toolkit · 20 stars · license AGPL-3.0 · pushed 2026-09-21 · branch main

API

GET https://markdownregistry.com/api/v1/artifacts/art_jrixkuxpvigfy3h7
GET https://markdownregistry.com/api/v1/resolve?ref=ammawla/encode-toolkit/regulatory-elements
GET https://markdownregistry.com/api/v1/blob/5d1993bfd3bfdb64640a8fd07717c28199e22c9b2f2e2a4e4fc7ac067aa3275c

Your agent does the legwork. You hear about the deals worth your word. Hand yours the standing instructions at modelranch.com and it joins the network that reads files like this one.

More from ammawla/encode-toolkit

accessibility-aggregation skill
ammawla/encode-toolkit · plugin/skills/accessibility-aggregation/SKILL.md · Build comprehensive chromatin accessibility maps by aggregating ATAC-seq and DNase-seq narrowPeak data across multiple…
git:20260312.665e731 · audit A · 20 stars
batch-analysis skill
ammawla/encode-toolkit · plugin/skills/batch-analysis/SKILL.md · Guide for multi-experiment batch operations: QC screening, batch download, comparison, and report generation across…
git:20260312.665e731 · audit A · 20 stars
bioinformatics-installer skill
ammawla/encode-toolkit · plugin/skills/bioinformatics-installer/SKILL.md · Install bioinformatics tools for ENCODE data analysis. Covers CLI tools (BWA, STAR, samtools, MACS2), R/Bioconductor…
git:20260321.5cfbcfd · audit B · 20 stars
cellxgene-context skill
ammawla/encode-toolkit · plugin/skills/cellxgene-context/SKILL.md · Guide for integrating CellxGene Census single-cell data with ENCODE bulk experiments. Use when users need…
git:20260321.5cfbcfd · audit A · 20 stars
cite-encode skill
ammawla/encode-toolkit · plugin/skills/cite-encode/SKILL.md · Generate proper ENCODE citations for publications, grants, and presentations. Use when the user needs to cite ENCODE…
git:20260312.665e731 · audit A · 20 stars
clinvar-annotation skill
ammawla/encode-toolkit · plugin/skills/clinvar-annotation/SKILL.md · Guide for annotating ENCODE regulatory variants with ClinVar clinical significance. Use when users need to check if…
git:20260312.665e731 · audit A · 20 stars
compare-biosamples skill
ammawla/encode-toolkit · plugin/skills/compare-biosamples/SKILL.md · Compare ENCODE experiments across different biosamples, tissues, or cell lines to identify tissue-specific regulatory…
git:20260312.665e731 · audit A · 20 stars
cross-reference skill
ammawla/encode-toolkit · plugin/skills/cross-reference/SKILL.md · Cross-reference ENCODE data with PubMed, bioRxiv, ClinicalTrials.gov, Open Targets, GTEx, ClinVar, GWAS Catalog…
git:20260312.665e731 · audit A · 20 stars
data-provenance skill
ammawla/encode-toolkit · plugin/skills/data-provenance/SKILL.md · Track exact provenance for every operation on ENCODE data — tool versions, reference files, scripts, parameters, and…
git:20260312.665e731 · audit A · 20 stars
disease-research skill
ammawla/encode-toolkit · plugin/skills/disease-research/SKILL.md · Use ENCODE functional genomics data for disease mechanism research. Use when the user wants to connect GWAS variants to…
git:20260321.5cfbcfd · audit A · 20 stars
download-encode skill
ammawla/encode-toolkit · plugin/skills/download-encode/SKILL.md · Download ENCODE genomics files (BED, FASTQ, BAM, bigWig, etc.) to the user's machine. Use when the user wants to…
git:20260321.5cfbcfd · audit A · 20 stars
ensembl-annotation skill
ammawla/encode-toolkit · plugin/skills/ensembl-annotation/SKILL.md · Query the Ensembl REST API for regulatory feature annotations, variant effect prediction (VEP), coordinate liftover…
git:20260312.665e731 · audit A · 20 stars

Every file in ammawla/encode-toolkit

Browse by kind, by grade A, or by owner.