Home / ammawla / encode-toolkit · plugin/skills/single-cell-encode/SKILL.md · GitHub

single-cell-encode skillA

single-cell-encode is agent-read markdown (skill) from ammawla/encode-toolkit: Find and work with ENCODE single-cell genomics data including scRNA-seq and scATAC-seq. Use when the user asks about single-cell experiments, cell type resolution, clustering from ENCODE data, deconvolution of bulk signals using single-cell references, or comparing single-cell vs bulk profiles. Covers platform differences (10X Chromium, Smart-seq2, Drop-seq), quality limitations of single-cell data, multimodal integration (RNA+ATAC), and cross-study reproducibility concerns. Also use for cell ty.

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What the file says

# Single-Cell ENCODE Data

## When to Use

- User wants to find or analyze single-cell data (scRNA-seq, scATAC-seq, snRNA-seq) from ENCODE
- User asks about "single-cell", "scRNA-seq", "scATAC-seq", "cell type annotation", or "single-nucleus"
- User needs to integrate ENCODE single-cell data with bulk epigenomic profiles
- User wants to identify cell-type-specific regulatory elements from single-cell chromatin accessibility
- Example queries: "find scRNA-seq data in ENCODE for brain", "what snATAC-seq is available?", "integrate single-cell with bulk ChIP-seq"

Help the user find and work with ENCODE single-cell genomics data, understand quality limitations relative to bulk assays, and integrate single-cell with bulk ENCODE profiles for cell-type-resolved regulatory analysis.

## Literature Foundation

| # | Reference | Key Contribution |
|---|-----------|-----------------|
| 1 | Mawla & Huising 2019, Endocrinology, DOI:10.1210/en.2018-01037 (~200 cit) | Cross-study scRNA-seq meta-analysis revealing that only ~1-2% of heterogeneity-driving genes replicate across studies; TIN-based quality assessment; detection-limit awareness framework. PMC6609986. |
…

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How to install

Latest version
mdr add ammawla/encode-toolkit/single-cell-encode@git:20260921.4ba10c6
Exact content
mdr add ammawla/encode-toolkit/single-cell-encode@sha256:5bbbf463798d14c8

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Versions

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git:20260921.4ba10c6 latest2026-09-21 4ba10c6 30,307 BA view · diff
git:20260920.60500032026-09-20 6050003 30,208 BA view · diff
git:20260920.ce7840d2026-09-20 ce7840d 29,092 BA view · diff
git:20260321.d9029d02026-03-21 d9029d0 29,125 BA view · diff
git:20260312.be327062026-03-12 be32706 29,195 BA view

Audit of the latest version

A  17 of 17 checks passed. Deterministic, no model, same answer every run.
  • pass: Frontmatter block present
  • pass: Frontmatter declares a name
  • pass: Frontmatter declares a description
  • pass: Size between 200 bytes and 200 KB (30307 bytes)
  • pass: No zero-width or bidi control characters
  • pass: No instruction hidden inside an HTML comment
  • pass: No link to an exfiltration or paste host
  • pass: No credential-shaped string
  • pass: No instruction to send local credentials anywhere
  • pass: No text hidden with inline styles
  • pass: No prompt-injection phrasing
  • pass: No curl or wget piped into a shell
  • pass: No recursive delete of root, home or parent
  • pass: No instruction to read or print local credentials
  • pass: No base64 blob over 200 characters
  • pass: No link to a raw IP address
  • pass: No script tag

Source

GitHub

ammawla/encode-toolkit · 20 stars · license AGPL-3.0 · pushed 2026-09-21 · branch main

API

GET https://markdownregistry.com/api/v1/artifacts/art_p2ooihc5ngip6srx
GET https://markdownregistry.com/api/v1/resolve?ref=ammawla/encode-toolkit/single-cell-encode
GET https://markdownregistry.com/api/v1/blob/5bbbf463798d14c8fef259bfc957126ff9958be2f288915fcc59de2f5dc88e0f

Your agent does the legwork. You hear about the deals worth your word. Hand yours the standing instructions at modelranch.com and it joins the network that reads files like this one.

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