Home / ammawla / encode-toolkit · plugin/skills/peak-annotation/SKILL.md · GitHub

peak-annotation skillA

peak-annotation is agent-read markdown (skill) from ammawla/encode-toolkit: Guide for annotating ENCODE peaks with genomic features using ChIPseeker and GREAT. Use when users need to assign peaks to genes, determine genomic feature distribution (promoter, intron, intergenic), or perform gene ontology enrichment of peak-associated genes. Trigger on: peak annotation, ChIPseeker, GREAT, peak to gene, genomic feature, promoter enrichment, gene ontology, peak distribution, TSS distance, nearest gene..

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What the file says

# Peak Annotation of ENCODE Data

## When to Use

- User wants to annotate genomic peaks with nearby genes, regulatory features, or functional categories
- User asks about "peak annotation", "ChIPseeker", "gene assignment", or "peak-to-gene mapping"
- User needs to classify peaks as promoter, enhancer, intronic, intergenic, etc.
- User wants to run GO/pathway enrichment on genes near their peaks
- Example queries: "annotate my H3K27ac peaks with nearby genes", "what genes are near these ATAC-seq peaks?", "run pathway analysis on peak-associated genes"

Help the user annotate ENCODE peak calls with genomic features and functional enrichment. Peak annotation bridges the gap between regulatory elements (peaks) and biological function (genes, pathways). This skill covers two complementary approaches: ChIPseeker for genomic feature annotation and visualization, and GREAT for functional enrichment analysis of non-coding regions.

## Literature Foundation

| Reference | Journal | Key Contribution | DOI | Citations |
|-----------|---------|-----------------|-----|-----------|
…

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How to install

Latest version
mdr add ammawla/encode-toolkit/peak-annotation@git:20260920.6050003
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Versions

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git:20260920.6050003 latest2026-09-20 6050003 21,126 BA view · diff
git:20260920.ce7840d2026-09-20 ce7840d 20,958 BA view · diff
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git:20260312.be327062026-03-12 be32706 20,971 BA view

Audit of the latest version

A  17 of 17 checks passed. Deterministic, no model, same answer every run.
  • pass: Frontmatter block present
  • pass: Frontmatter declares a name
  • pass: Frontmatter declares a description
  • pass: Size between 200 bytes and 200 KB (21126 bytes)
  • pass: No zero-width or bidi control characters
  • pass: No instruction hidden inside an HTML comment
  • pass: No link to an exfiltration or paste host
  • pass: No credential-shaped string
  • pass: No instruction to send local credentials anywhere
  • pass: No text hidden with inline styles
  • pass: No prompt-injection phrasing
  • pass: No curl or wget piped into a shell
  • pass: No recursive delete of root, home or parent
  • pass: No instruction to read or print local credentials
  • pass: No base64 blob over 200 characters
  • pass: No link to a raw IP address
  • pass: No script tag

Source

GitHub

ammawla/encode-toolkit · 20 stars · license AGPL-3.0 · pushed 2026-09-21 · branch main

API

GET https://markdownregistry.com/api/v1/artifacts/art_uqg5rtnto72okhmf
GET https://markdownregistry.com/api/v1/resolve?ref=ammawla/encode-toolkit/peak-annotation
GET https://markdownregistry.com/api/v1/blob/9c26b29e3ef8de635214c6ea84a5989c529f10e12a74e1206523b0a76d914072

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