sgrna-design-guide SKILL.md skillA
sgrna-design-guide from jaechang-hits/sciagent-skills is an agent skill: a SKILL.md folder an AI agent loads when a task calls for it.
mdr add jaechang-hits/sciagent-skills/sgrna-design-guide@git:20260502.5e39ba9Pins git:20260502.5e39ba9 in mdr.lock. Pin the fingerprint instead.
- Audit grade
- A, 17 of 17 checks passed
- Kind
- SKILL.md skill
- Repository
- jaechang-hits/sciagent-skills
- Path
skills/molecular-biology/sgrna-design-guide/SKILL.md- Versions recorded
- 3
- Latest version
- git:20260502.5e39ba9, committed May 2, 2026
- First version recorded
- Feb 18, 2026
- Size
- 24,817 bytes
- Repository stars
- 381
- License
- a license file GitHub does not identify
Three-tiered sgRNA design guide using validated Addgene sequences, CRISPick pre-computed datasets, or de novo design rules for CRISPR experiments.
Collected from public GitHub. Every version is saved exactly as it was and never changes. Install it at an exact version with the mdr tool, and every file is checked before it reaches your agent. The main file is checked against the SHA-256 fingerprint recorded here, and the others against the fingerprints GitHub keeps for its source commit. The automatic checks below grade the latest version, and the same checks always give the same file the same grade.
What the file says
# sgRNA Design Guide: Three-Tiered Approach --- ## Metadata **Short Description**: Comprehensive guide for finding or designing sgRNAs using validated sequences, CRISPick datasets, or de novo design tools. **Authors**: Ohagent Team **Version**: 1.0 **Last Updated**: November 2025 **License**: CC BY 4.0 **Commercial Use**: Allowed ## Citations and Acknowledgments ### If you use validated sgRNAs from our database (Option 1): - **Database Source**: Addgene (https://www.addgene.org) - **Citation**: Always cite the original publication associated with each sgRNA using the PubMed ID provided in the database - **Acknowledgment**: "Validated sgRNA sequences obtained from Addgene (https://www.addgene.org/crispr/reference/grna-sequence/)" ### If you use CRISPick designs (Option 2): - **Acknowledgment Statement**: "Guide designs provided by the CRISPick web tool of the GPP at the Broad Institute" - **Citation for Cas9 designs (SpCas9, SaCas9)**: Sanson KR, et al. Optimized libraries for CRISPR-Cas9 genetic screens with multiple modalities. Nat Commun. 2018;9(1):5416. PMID: 30575746 …
Read the whole file at its exact version.
How to install
mdr add jaechang-hits/sciagent-skills/sgrna-design-guide@sha256:11951ebc7c6c0526The line at the top of this page pins the label git:20260502.5e39ba9, which is what most people want. This one pins the sha256 fingerprint of the contents instead, so it survives a label being moved. Either way the exact version is written to mdr.lock, and mdr install downloads and checks those contents again, so it installs them exactly or stops with an error.
[](https://markdownregistry.com/a/art_wmzcyv7iost3t7kj)
2 badge image fetches in 30 days. What the badge shows.
Versions
The registry has recorded 3 versions of this file, between Feb 18, 2026 and May 2, 2026, about one every 36 days.
| version | committed | commit | size | audit | |
|---|---|---|---|---|---|
| git:20260502.5e39ba9 latest | 2026-05-02 | 5e39ba9 | 24,817 B | A | view · diff |
| git:20260428.81ea2d2 | 2026-04-28 | 81ea2d2 | 20,116 B | A | view · diff |
| git:20260218.8df1694 | 2026-02-18 | 8df1694 | 20,310 B | A | view |
Audit of the latest version
- pass: Frontmatter block present
- pass: Frontmatter declares a name
- pass: Frontmatter declares a description
- pass: Size between 200 bytes and 200 KB (24817 bytes)
- pass: No zero-width or bidi control characters
- pass: No instruction hidden inside an HTML comment
- pass: No link to an exfiltration or paste host
- pass: No credential-shaped string
- pass: No instruction to send local credentials anywhere
- pass: No text hidden with inline styles
- pass: No prompt-injection phrasing
- pass: No curl or wget piped into a shell
- pass: No recursive delete of root, home or parent
- pass: No instruction to read or print local credentials
- pass: No base64 blob over 200 characters
- pass: No link to a raw IP address
- pass: No script tag
Source
jaechang-hits/sciagent-skills · 381 stars · license a license file GitHub does not identify · pushed 2026-10-16 · branch main
API
GET https://markdownregistry.com/api/v1/artifacts/art_wmzcyv7iost3t7kj GET https://markdownregistry.com/api/v1/resolve?ref=jaechang-hits/sciagent-skills/sgrna-design-guide GET https://markdownregistry.com/api/v1/blob/11951ebc7c6c0526075526cf296247fbe17390b2975d34e23091b5d514e2384b
Your AI assistant does the legwork. You hear about the deals worth your word. Hand yours the standing instructions at modelranch.com and it joins the network that reads files like this one.
Guides: the SKILL.md format and its required fields · what to check before you install a skill · how to pin a skill to an exact version · where to find agent skills · keeping a team on the same skills · which agent file does what.