opentrons-protocol-api SKILL.md skillA

opentrons-protocol-api from jaechang-hits/sciagent-skills is an agent skill: a SKILL.md folder an AI agent loads when a task calls for it.

Install it, pinned
mdr add jaechang-hits/sciagent-skills/opentrons-protocol-api@git:20260428.81ea2d2

Pins git:20260428.81ea2d2 in mdr.lock. Pin the fingerprint instead.

Audit grade
A, 17 of 17 checks passed
Kind
SKILL.md skill
Repository
jaechang-hits/sciagent-skills
Path
skills/lab-automation/opentrons-protocol-api/SKILL.md
Versions recorded
2
Latest version
git:20260428.81ea2d2, committed Apr 28, 2026
First version recorded
Feb 18, 2026
Size
30,944 bytes
Repository stars
381
License
a license file GitHub does not identify

Python API v2 for Opentrons OT-2/Flex liquid handlers: protocols as Python files with metadata and run(); control pipettes, labware, and modules (thermocycler, heater-shaker, magnetic, temperature). Simulate via opentrons_simulate then upload. Use PyLabRobot for vendor-agnostic scripts (Hamilton, Tecan).

Collected from public GitHub. Every version is saved exactly as it was and never changes. Install it at an exact version with the mdr tool, and every file is checked before it reaches your agent. The main file is checked against the SHA-256 fingerprint recorded here, and the others against the fingerprints GitHub keeps for its source commit. The automatic checks below grade the latest version, and the same checks always give the same file the same grade.

What the file says

# Opentrons Python Protocol API

## Overview

The Opentrons Protocol API v2 lets you write liquid handling protocols as plain Python files that run on OT-2 or Flex robots. Every protocol defines a `metadata` dictionary, an optional `requirements` dictionary, and a `run(protocol)` function. The `ProtocolContext` object passed to `run()` exposes all deck setup, pipette operations, module control, and utility methods. Protocols can be simulated on any computer with `opentrons_simulate` before uploading to the robot through the Opentrons App or HTTP API.

## When to Use

- **Setting up PCR reactions**: Distribute master mix from a tube rack into a thermocycler plate, add template DNA from individual tubes, then execute a PCR profile automatically.
- **Running serial dilutions**: Programmatically step a multi-channel pipette across a 96-well plate to create 2-fold or custom dilution curves with defined diluent volumes.
- **Performing ELISA plate layouts**: Add blocking buffer, primary antibody, secondary antibody, and substrate to defined wells with tip changes between each reagent.
…

Read the whole file at its exact version.

How to install

The exact contents, by fingerprint
mdr add jaechang-hits/sciagent-skills/opentrons-protocol-api@sha256:d4b71f9b471205ee

The line at the top of this page pins the label git:20260428.81ea2d2, which is what most people want. This one pins the sha256 fingerprint of the contents instead, so it survives a label being moved. Either way the exact version is written to mdr.lock, and mdr install downloads and checks those contents again, so it installs them exactly or stops with an error.

Badge

mdr badge

[![mdr](https://markdownregistry.com/badge/art_zxnb5zuus6pmoz66.svg)](https://markdownregistry.com/a/art_zxnb5zuus6pmoz66)

3 badge image fetches in 30 days. What the badge shows.

Versions

The registry has recorded 2 versions of this file, between Feb 18, 2026 and Apr 28, 2026, about one every 69 days.

versioncommittedcommitsizeaudit
git:20260428.81ea2d2 latest2026-04-28 81ea2d2 30,944 BA view · diff
git:20260218.8df16942026-02-18 8df1694 31,058 BA view

Audit of the latest version

A  17 of 17 checks passed. Fixed automatic checks with no AI involved, so the same checks give the same answer every run.
  • pass: Frontmatter block present
  • pass: Frontmatter declares a name
  • pass: Frontmatter declares a description
  • pass: Size between 200 bytes and 200 KB (30944 bytes)
  • pass: No zero-width or bidi control characters
  • pass: No instruction hidden inside an HTML comment
  • pass: No link to an exfiltration or paste host
  • pass: No credential-shaped string
  • pass: No instruction to send local credentials anywhere
  • pass: No text hidden with inline styles
  • pass: No prompt-injection phrasing
  • pass: No curl or wget piped into a shell
  • pass: No recursive delete of root, home or parent
  • pass: No instruction to read or print local credentials
  • pass: No base64 blob over 200 characters
  • pass: No link to a raw IP address
  • pass: No script tag

Source

GitHub

jaechang-hits/sciagent-skills · 381 stars · license a license file GitHub does not identify · pushed 2026-10-16 · branch main

API

GET https://markdownregistry.com/api/v1/artifacts/art_zxnb5zuus6pmoz66
GET https://markdownregistry.com/api/v1/resolve?ref=jaechang-hits/sciagent-skills/opentrons-protocol-api
GET https://markdownregistry.com/api/v1/blob/d4b71f9b471205eee737ebe2367e83c883e74170de71cabbb98bcc651b5b8513

Your AI assistant does the legwork. You hear about the deals worth your word. Hand yours the standing instructions at modelranch.com and it joins the network that reads files like this one.

Guides: the SKILL.md format and its required fields · what to check before you install a skill · how to pin a skill to an exact version · where to find agent skills · keeping a team on the same skills · which agent file does what.

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