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bio-alignment-trimming skillA

bio-alignment-trimming is agent-read markdown (skill) from pku-yuangroup/openai4s: Trim multiple sequence alignments using ClipKIT, trimAl, BMGE, Divvier, or HMMcleaner with mode selection guidance per downstream goal. Use when removing unreliable columns or contaminating residues before phylogenetic inference, HMM building, or selection analysis..

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What the file says

## Version Compatibility

Reference examples tested with: ClipKIT 2.1+, trimAl 1.4+, BMGE 1.12+, Divvier 1.01+, HMMcleaner (current CPAN release of `Bio::MUST::Apps::HmmCleaner`), BioPython 1.83+

Before using code patterns, verify installed versions match. If versions differ:
- CLI: `clipkit --version`, `trimal --version`, `BMGE --help`, `Divvier --help`
- Python: `pip show <package>` then `help(module.function)` to check signatures

If code throws ImportError, AttributeError, or TypeError, introspect the installed package and adapt the example to match the actual API rather than retrying.

# Alignment Trimming

**"Remove unreliable columns from this MSA"** -> Filter or split columns based on gap fraction, conservation, entropy, or per-residue quality.
- CLI: `clipkit`, `trimal`, `BMGE`, `Divvier`, `HMMcleaner`
- Python: post-process via Bio.AlignIO with custom column masks

**"Make this alignment publication-grade for phylogenetics"** -> Apply ClipKIT's `kpic-smart-gap` mode, or trimAl `-automated1`, then verify via tree-stability comparison before vs after trimming.
…

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A  17 of 17 checks passed. Deterministic, no model, same answer every run.
  • pass: Frontmatter block present
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Source

GitHub

pku-yuangroup/openai4s · 586 stars · license MIT · pushed 2026-09-23 · branch main

API

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