bio-crispr-screens-combinatorial-screens SKILL.md skillA
bio-crispr-screens-combinatorial-screens from pku-yuangroup/openai4s is an agent skill: a SKILL.md folder an AI agent loads when a task calls for it.
mdr add pku-yuangroup/openai4s/bio-crispr-screens-combinatorial-screens@git:20260821.2d1b678Pins git:20260821.2d1b678 in mdr.lock. Pin the fingerprint instead.
- Audit grade
- A, 17 of 17 checks passed
- Kind
- SKILL.md skill
- Repository
- pku-yuangroup/openai4s
- Path
skills/bioskills/bio-crispr-screens-combinatorial-screens/SKILL.md- Versions recorded
- 1
- Latest version
- git:20260821.2d1b678, committed Aug 21, 2026
- Size
- 18,272 bytes
- Repository stars
- 662
- License
- MIT
Designs and analyzes combinatorial CRISPR screens covering paired-Cas9 (Big Papi, Najm 2018), enhanced AsCas12a multiplex (enCas12a, DeWeirdt 2021), in4mer 4-guide-array Cas12a (Esmaeili Anvar N et al 2024 Nat Commun 15:3577) and the Inzolia paralog-pair library, paralog-buffering detection (Dede 2020 Genome Biol; Thompson 2021 Nat Commun 12:1302), genetic-interaction (GI) scoring as observed_double_LFC minus expected_additive_double_LFC, synthetic-lethal and synthetic-rescue interaction…
Collected from public GitHub. Every version is saved exactly as it was and never changes. Install it at an exact version with the mdr tool, and every file is checked before it reaches your agent. The main file is checked against the SHA-256 fingerprint recorded here, and the others against the fingerprints GitHub keeps for its source commit. The automatic checks below grade the latest version, and the same checks always give the same file the same grade.
What the file says
## Version Compatibility Reference examples tested with: MAGeCK 0.5.9+ (for MLE with interaction terms), Inzolia library annotation (Esmaeili Anvar 2024), pandas 2.2+, numpy 1.26+, scipy 1.12+, matplotlib 3.8+. Before using code patterns, verify installed versions match. If versions differ: - CLI: `mageck --version`; `mageck mle --help` - For Cas12a libraries: verify against published Inzolia / in4mer / Big Papi annotations If code throws ImportError, AttributeError, or TypeError, introspect the installed package and adapt the example to match the actual API rather than retrying. ## Combinatorial CRISPR Screen Analysis **"Run a combinatorial CRISPR screen to find synthetic-lethal interactions"** -> Design a paired or multiplex library, screen for double-knockout fitness, score per-pair genetic interaction (GI = observed_double - expected_additive), and identify synthetic-lethal (negative GI) and synthetic-rescue (positive GI) interactions. - CLI: `mageck mle` with explicit interaction terms for paired-Cas9 (Big Papi-style) - Python: custom GI scoring for Cas12a multiplex (in4mer / Inzolia) - Modality: enCas12a / LbCas12a single-array multiplex (preferred for paralog screens) …
Read the whole file at its exact version.
How to install
mdr add pku-yuangroup/openai4s/bio-crispr-screens-combinatorial-screens@sha256:cddb4a95d7f2badfThe line at the top of this page pins the label git:20260821.2d1b678, which is what most people want. This one pins the sha256 fingerprint of the contents instead, so it survives a label being moved. Either way the exact version is written to mdr.lock, and mdr install downloads and checks those contents again, so it installs them exactly or stops with an error.
[](https://markdownregistry.com/a/art_c7qsq7m6j3oxcezk)
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Versions
The registry has recorded 1 version of this file, committed on Aug 21, 2026.
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Source
pku-yuangroup/openai4s · 662 stars · license MIT · pushed 2026-11-03 · branch main
API
GET https://markdownregistry.com/api/v1/artifacts/art_c7qsq7m6j3oxcezk GET https://markdownregistry.com/api/v1/resolve?ref=pku-yuangroup/openai4s/bio-crispr-screens-combinatorial-screens GET https://markdownregistry.com/api/v1/blob/cddb4a95d7f2badf35d7ac1ade1e87e1b7eb8005c87797d793c41f94163ce0df
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