bio-crispr-screens-prime-editing-screens SKILL.md skillA
bio-crispr-screens-prime-editing-screens from pku-yuangroup/openai4s is an agent skill: a SKILL.md folder an AI agent loads when a task calls for it.
mdr add pku-yuangroup/openai4s/bio-crispr-screens-prime-editing-screens@git:20260821.2d1b678Pins git:20260821.2d1b678 in mdr.lock. Pin the fingerprint instead.
- Audit grade
- A, 17 of 17 checks passed
- Kind
- SKILL.md skill
- Repository
- pku-yuangroup/openai4s
- Path
skills/bioskills/bio-crispr-screens-prime-editing-screens/SKILL.md- Versions recorded
- 1
- Latest version
- git:20260821.2d1b678, committed Aug 21, 2026
- Size
- 17,163 bytes
- Repository stars
- 662
- License
- MIT
Designs and analyzes pooled prime-editor (PE) screens for installing precise genetic variants without bystander confounding. Covers pegRNA design with PRIDICT and PRIDICT2 for predicting per-pegRNA editing efficiency, pegRNA architecture (spacer + scaffold + PBS + RTT), PE2/PE3/PE3b/PEmax variants, MOSAIC in situ saturation mutagenesis, the PRIME pooled-screen methodology (Ren 2023; ~3,699 ClinVar variant screens), chromatin context as a major locus-level determinant of PE efficiency…
Collected from public GitHub. Every version is saved exactly as it was and never changes. Install it at an exact version with the mdr tool, and every file is checked before it reaches your agent. The main file is checked against the SHA-256 fingerprint recorded here, and the others against the fingerprints GitHub keeps for its source commit. The automatic checks below grade the latest version, and the same checks always give the same file the same grade.
What the file says
## Version Compatibility Reference examples tested with: PRIDICT2 v1.0+ (https://github.com/uzh-dqbm-cmi/PRIDICT2), CRISPResso2 2.2.14+, pandas 2.2+, biopython 1.83+, numpy 1.26+. Before using code patterns, verify installed versions match. If versions differ: - CLI: `python pridict2_pegRNA_design.py single --help`; `python pridict2_pegRNA_design.py batch --help` - Web: PRIDICT2 web interface at https://pridict.it/ If code throws ImportError, AttributeError, or TypeError, introspect the installed package and adapt the example to match the actual API rather than retrying. ## Prime-Editing Screen Analysis **"Design or analyze a pooled prime-editor screen"** -> Design pegRNAs (spacer + scaffold + PBS + RTT) for intended edits, predict efficiency with PRIDICT2, filter pre-synthesis to efficient candidates, install variants in the screen, quantify intended-edit vs scaffold-incorporation vs indel via CRISPResso2, and aggregate to per-variant fitness scores. - Python: `PRIDICT2` for pegRNA efficiency prediction - Python: `ePRIDICT` for chromatin-context prediction; pair with PRIDICT2 rather than replacing it - CLI: `CRISPResso --prime_editing_pegRNA_*` for amplicon-level analysis …
Read the whole file at its exact version.
How to install
mdr add pku-yuangroup/openai4s/bio-crispr-screens-prime-editing-screens@sha256:d170846b9c10623aThe line at the top of this page pins the label git:20260821.2d1b678, which is what most people want. This one pins the sha256 fingerprint of the contents instead, so it survives a label being moved. Either way the exact version is written to mdr.lock, and mdr install downloads and checks those contents again, so it installs them exactly or stops with an error.
[](https://markdownregistry.com/a/art_j2jqg372erpcugpl)
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Versions
The registry has recorded 1 version of this file, committed on Aug 21, 2026.
Audit of the latest version
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Source
pku-yuangroup/openai4s · 662 stars · license MIT · pushed 2026-11-03 · branch main
API
GET https://markdownregistry.com/api/v1/artifacts/art_j2jqg372erpcugpl GET https://markdownregistry.com/api/v1/resolve?ref=pku-yuangroup/openai4s/bio-crispr-screens-prime-editing-screens GET https://markdownregistry.com/api/v1/blob/d170846b9c10623a7b37ff191fcc548ecb9a857ed6fa962456073ed0ce6ae8d2
Your AI assistant does the legwork. You hear about the deals worth your word. Hand yours the standing instructions at modelranch.com and it joins the network that reads files like this one.
Guides: the SKILL.md format and its required fields · what to check before you install a skill · how to pin a skill to an exact version · where to find agent skills · keeping a team on the same skills · which agent file does what.