Home / pku-yuangroup / openai4s · skills/bioskills/bio-alignment-msa-parsing/SKILL.md · GitHub

bio-alignment-msa-parsing skillA

bio-alignment-msa-parsing is agent-read markdown (skill) from pku-yuangroup/openai4s: Parse and analyze multiple sequence alignments using Biopython. Extract sequences, identify conserved regions, analyze gaps, work with annotations, and manipulate alignment data for downstream analysis. Use when parsing or manipulating multiple sequence alignments..

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What the file says

## Version Compatibility

Reference examples tested with: BioPython 1.83+, numpy 1.26+

Before using code patterns, verify installed versions match. If versions differ:
- Python: `pip show <package>` then `help(module.function)` to check signatures

If code throws ImportError, AttributeError, or TypeError, introspect the installed
package and adapt the example to match the actual API rather than retrying.

# MSA Parsing and Analysis

Parse multiple sequence alignments to extract information, analyze content, and prepare for downstream analysis.

## Required Import

**Goal:** Load modules for parsing, analyzing, and manipulating multiple sequence alignments.

**Approach:** Import AlignIO for reading, Counter for column analysis, and alignment classes for constructing modified alignments.

```python
from Bio import AlignIO
from Bio.Align import MultipleSeqAlignment
from Bio.SeqRecord import SeqRecord
from Bio.Seq import Seq
from collections import Counter
import numpy as np
import pandas as pd
```

Optional for streaming and Easel-based weighting:
```python
import pyhmmer
```

## Loading Alignments

**Goal:** Read an MSA file and inspect its dimensions.
…

Read the whole file at its exact version.

How to install

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Source

GitHub

pku-yuangroup/openai4s · 586 stars · license MIT · pushed 2026-09-23 · branch main

API

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GET https://markdownregistry.com/api/v1/resolve?ref=pku-yuangroup/openai4s/bio-alignment-msa-parsing
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